Nonenzymatically glycated albumin (Amadori adducts) enhances nitric oxide synthase activity and gene expression in endothelial cells

EXPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE GENE (iNOS) STIMULATED BY HYDROGEN PEROXIDE IN HUMAN ENDOTHELIAL CELLS

Inducible nitric oxide synthase (iNOS) gene expresses a calcium calmudolin-independent enzyme which can catalyse NO production from L-arginine. The induction of iNOS activity has been demonstrated in a wide variety of cell types under stimulation with cytokines and lipopoly saccharide (LPS). Previous studies indicated that all nitric oxide synthases (NOS) activated in human umbilical vein endot...

Vascular smooth muscle cell activation by glycated albumin (Amadori adducts).

Nonenzymatic glycation is increased in diabetes. The role of advanced glycation end products has been implicated in many of the complications of diabetes, whereas the effects of early-glycation Amadori-modified proteins on vascular cells alone are poorly defined. In the present study, we show that glycated serum albumin (GSA) induces a parallel activation of the redox-responsive transcription f...

expression of inducible nitric oxide synthase gene (inos) stimulated by hydrogen peroxide in human endothelial cells

inducible nitric oxide synthase (inos) gene expresses a calcium calmudolin-independent enzyme which can catalyse no production from l-arginine. the induction of inos activity has been demonstrated in a wide variety of cell types under stimulation with cytokines and lipopoly saccharide (lps). previous studies indicated that all nitric oxide synthases (nos) activated in human umbilical vein endot...

Association of endothelial nitric oxide synthase gene G894T polymorphism with type two diabetes and diabetic nephropathy

Background: Nitric oxide (NO) produced by endothelial NO synthase (eNOS) mediates a large range of processes, and abnormality in the production of NO has been implicated in diabetic complications including diabetic nephropathy (DN). G894T polymorphism in the eNOS gene has been shown to decreased activity the NO levels of plasma. The association between eNOS Glu298Asp gene polymorphism and DN ri...

Tetrahydrobiopterin and endothelial nitric oxide synthase activity.